Metabolite screening

Non-targeted screening

We use a comprehensive non-targeted profiling technique for the analysis of biological samples. Relative abundance measurements of analytes are used to perform differential analysis across samples/sample classes using fold change, statistics and/or visualisation analysis. This type of non-targeted approach provides insight into metabolite changes, perturbed metabolite pathways and/or chemical markers.

Services available

• Profiling for differential metabolites
• Chemical markers
• Volatile (aroma) profile
• Non-volatile aroma profile
  • polar metabolites, and/or
  • mid-polarity metabolites.

Deliverables resulting from services carried out include:

• Bioinformatics for data extraction/processing
• Sample data matrix of retention time. vs. relative abundance
• Raw data files from the entire batch (if required)
• Identification of metabolite based on RT/MS data from standards (AWRI in–house library)
• Descriptive statistics (average response, %CV)
• Multivariate analysis (if applicable).